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. 2019 Aug 12;9:11587. doi: 10.1038/s41598-019-47734-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Essential role of sEV in cell migration of TAMR-MCF-7 cells. (A) Migration of TAMR-MCF-7 cells after incubation with TAMR-MCF-7 sEV (16 and 50 μg/mL) for 18 h (n = 10). (B) Migration of TAMR-MCF-7 cells after incubation with MCF-7 or TAMR-MCF-7 sEV (1.6 and 16 μg/mL) for 18 (n = 10). (C) Migration of MCF-7 cells after incubation with MCF-7 or TAMR-MCF-7 sEV (1.6 and 16 μg/mL) for 18 h (n = 10). Cell migration was evaluated by Transwell migration assay in sEV-depleted FBS condition. (D) Migration of T47D and TAMR-MCF-7 cells after incubation with T47D and TAMR-MCF-7 sEV (15 μg/mL) for 24 h (n = 3). Migrated cells were marked in red circle by image analysis. Images were taken by Incucyte Zoom. All data represent the mean ± SE (^###p < 0.005, significant as compared to TAMR-MCF-7 cells in sEV depleted condition, ⁺⁺p < 0.01, significant as compared to TAMR-MCF-7 cells in T47D sEV 15 μg/mL condition).