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. 2019 Aug 12;9:11649. doi: 10.1038/s41598-019-48204-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nanostring quantification of globin gene expression in erythroid cells after treatment with vorinostat. Erythroid cells differentiated from human adult CD34⁺ cells were incubated with vorinostat (500 nM) or DMSO (control) for 72 hours on day 7 of the differentiation and the globin mRNA levels were quantified using Nanostring nCounter digital analyser. Mean Nanostring counts of globin genes normalized to the mean of multiple housekeeping genes (RPL13A, RPL18, GAPDH, PABPC1, CA2, FTH1, PAIP2 and LAPTM4A) from 3 independent biological repeats is shown; error bars represent SD; *p < 0.05, **p < 0.01, ***p < 0.001 relative to DMSO control. Scale of the y-axis is logarithmic (base-2).