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. 2019 Aug 12;9:11657. doi: 10.1038/s41598-019-48106-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Whole genome sequence analysis of SNPs of M. bovis isolates. (A) A phylogenetic tree inferred using the neighbor-joining method based on the predicted SNPs of each isolate compared with the M. bovis AF2122/97 reference genome. Numbers at each branch represent bootstrap values and branches correspond to partitions with >50% bootstrap replicates. The tree is drawn to a scale with evolutionary distances computed using the maximum composite likelihood method in MEGA7. (B) Histogram of the number of SNPs (synonymous, non-synonymous and intergenic) for each examined M. bovis isolate compared to the reference genome.