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. 2019 Aug 12;9:11585. doi: 10.1038/s41598-019-47737-w

Figure 2.

Figure 2

Spheroids show no morphological changes for up to 10 days of FA treatment. Bright-field images of spheroids from the TM6SF2 E167K-2 (A), WT-1 (B) and WT-2 (C) donor, without (top row, Control, FA-free BSA) and with FA treatment (bottom row, high FA, BSA + 213 µM OA/107 µM PA) for up to 10 days showed no morphological differences between the two populations. Corresponding bright-field images of the TM6SF2 E167K-1 and WT-3 donors are shown in Supplementary Fig. S3. Bar = 200 µm. Abbreviations: BSA, bovine serum albumin; FA, fatty acid; OA, oleic acid; PA, palmitic acid; WT, wild type.