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. 2019 Aug 12;9:11585. doi: 10.1038/s41598-019-47737-w

Figure 3.

Figure 3

Spheroid viability is not compromised by FA treatment. Total intracellular ATP content measured by luminescence using CellTiter-Glo® 3D Cell Viability Assay (Promega) in primary human hepatocyte spheroids with and without FA treatment for up to 10 days, using control (FA-free BSA) and two FA concentrations (low FA, BSA + 107 µM OA/53 µM PA and high FA, BSA + 213 µM OA/107 µM PA). Spheroids were generated from hepatocytes derived from five individual donors, out of which two were mutant and three were wild type for TM6SF2 E167K. Data are mean ± SD of eight single spheroid replicates per time point and condition and from one spheroid preparation per donor. Abbreviations: ATP, adenosine triphosphate; BSA, bovine serum albumin; FA, fatty acid; OA, oleic acid; PA, palmitic acid.