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. 2019 Aug 12;9:11585. doi: 10.1038/s41598-019-47737-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spheroids are well-differentiated for up to 10 days of fatty acid incubation. Intracelluar (A) and extracellular (B) APOB protein levels in spheroids and culture medium, respectively, up to 10 days of FA incubation. APOB was measured by immunoblotting analysis, using anti-APOB and anti-calnexin (CNX) antibodies, from cell lysate and medium supernatant of primary human hepatocyte spheroids that were derived from three individual donors. APOB levels were assessed with low (BSA + 107 µM OA/53 µM PA), high (BSA + 213 µM OA/107 µM PA) and without (FA-free BSA) incubation with FAs. Original, uncropped blots are presented in Supplementary Fig. S8. This figure shows data from of one independent experiment per donor. Abbreviations: Ab’, antibody; APOB, APOLIPOPROTEINB100; BSA, bovine serum albumin; CNX, Calnexin; FA, fatty acid.