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. 2019 Aug 12;9:11628. doi: 10.1038/s41598-019-48169-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Results of apoptosis assay in MDR KB/VIN cells. KB/VIN were treated with culture medium (a), danazol (b), β-estradiol (c), and deoxycorticosterone (d) for 24, 48, and 72 hours. Apoptosis and necrosis status of each sample was determined by annexin V (X-axis FITC) and PI (Y-axis PI). Cells distributed in Q1, Q2, Q3, and Q4 represented necrosis, late-apoptosis, normal, and early-apoptosis, respectively. Danazol, β-estradiol, and deoxycorticosterone exhibited prominent cell early-apoptosis after 72 hours treatment.