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. 2019 Aug 12;9:11695. doi: 10.1038/s41598-019-48081-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fold regulation of human liver miRNAs in HCC cells. (A) PLC/PRF/5 Parental vs PLC/PRF/5 EveR and PLC/PRF/5 EveR vs PLC/PRF/5 EveR + VitD; (B) JHH-6 Parental vs JHH-6 EveR and JHH-6 EveR vs JHH-6 EveR + VitD. Generated scatter plot compares the normalized expression of every miRNA on the array between two groups by plotting them against one another to quickly visualize large miRNA expression changes. The central line indicates unchanged miRNAs expression. The lateral dotted lines represent the selected boundaries. miRNAs whose expression changes are greater than the selected boundaries will be listed as significant up- or down- regulated miRNAs. Yellow dots represent up-regulated miRNAs while blue dots the down-regulated miRNAs. Dot in the circle represents miR-375.