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. 2019 Aug 12;9:11632. doi: 10.1038/s41598-019-48064-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SH3GL2 mediates rescue or abrogation of SPANXB1 expression and are physically associated with SPANXB1. (A) Silencing or overexpression of SH3GL2 (B) rescue or diminishes SPANXB1 expression in SPANXB1-KD or overexpressing cells respectively. (C) Bioinformatics analysis predicted possible interaction sites (green areas) between SPANXB1 and SH3GL2. (D) Co-IP analysis pulled down SPANXB1 with SH3GL2 or vice versa in SPANXB1 overexpressing HMLE cells. (E) High nuclear (black arrow) and cytoplasmic (red arrow) expression of SPANXB1 in primary cancer and matched lymph node metastatic tissues compared to normal controls (p = 0.0002–0.0001). Magnification x 200. (F,G) High SPANXB1 mRNA expression in overall BCa (p < 0.0001, (F)) and TNBC tissues (p < 0.0001, (G)) compared to normal in TCGA datasets, with distribution of data points plotted on top of box plots. Actin was used as a loading control. CSiRNA: Control SIRNA; SiRNA-SH3GL2: SH3GL2 targeted SiRNA pool. EV: Empty vector treated; SH3GL2: SH3GL2 transduced. In Western blotting, all experimental and control antibodies were run in parallel for the same immunoblot. The Image J software (https://imagej.nih.gov/ij/) was used for Western blot quantification.