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. 2019 Aug 12;9:11632. doi: 10.1038/s41598-019-48064-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SPANXB1 depleted TNBC cells failed to achieve robust primary tumor growth and distant metastasis. (A) Markedly reduced (p = 0.0005–0.006) primary tumor growth of the SPANXB1-deficient MDA-MB-231 cells in vivo compared to the controls. (B) Low SPANXB1:SH3GL2 expression ratio in the SPANXB1-deficient primary TNBC tumors. (C) Reduced pulmonary metastasis of the SPANXB1-deficient TNBC cells compared to the controls. The number of metastatic lung nodules was lower in the SPANXB1-deficient mice group compared to the control groups. (D) Absence of visible or microscopic liver metastasis of the orthotopically implanted SPANXB1-deficient TNBC cells in the NSG mice. Control cells achieve extensive liver metastasis (arrows). CSi: Control SiRNA treated; KD: SPANXB1-SiRNA treated. Actin was used as a loading control (B). CSi: M: Mouse. CSi: Control SiRNA; KD: SPANXB1-SiRNA treated. Magnification X 200 (C). In Western blotting, all experimental and control antibodies were run in parallel for the same immunoblot. The Image J software (https://imagej.nih.gov/ij/) was used for Western blot quantification.