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. 2019 Aug 12;9:11680. doi: 10.1038/s41598-019-48285-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunotoxin stability at physiological conditions. Different aliquots of IMTXCEAαS and IMTXTRICEAαS were incubated with FBS at 37 °C for up to 96 hours. Aliquots were taken every 24 h and analyzed. (A) Western blot analysis using an anti-α-sarcin antisera. Cropped-blots are displayed. Full-length blots are presented in Supplementary Fig. S5A. Blot images were acquired and analyzed using the ChemiDoc-It (UVP) and VisionWorks LS (B) Ribonucleolytic activity assays. IMTXCEAαS gel displayed was cropped and reorganized from the original gel. Gel image was acquired and analyzed using the Gel Doc XR Imaging System and Quantity One 1-D analysis software (BioRad). The original full-length gel is presented in Supplementary Fig. S3B. (C) Flow cytometry study of immunotoxins binding to SW1222 cells.