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. 2019 Aug 12;9:11614. doi: 10.1038/s41598-019-47950-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression patterns and distribution of drought responsive NLR genes in Picea glauca. (a) Heatmap illustrating the 14 expression groups formed of the 119 NLR sequences with drought responsive transcript levels for each genotype, treatment and sampling day. Red asterisks indicate expression groups with a single subfamily of NLR. (b) Number of drought responsive NLR genes and Log2 Fold Change observed in the four subfamilies and undetermined (U). (c) Measurement of water potential in needles for control and water-stressed plants at five sampling times. Each point is the average of 12 biological replicates (3 P. glauca genotypes with 4 replicates each) with standard deviation (adapted from Stival Sena et al.⁴⁰).