Figure 4.
Confirmation of results generated by CytoBackBone. (A) Leukocytes were collected from HIV-infected patients and healthy subjects. Cells were stained with a single antibody panel and SPADE analysis was performed to isolate monocytes. The biplot representations show the co-expression of CD64 and TLR2, CD11b and CD32, and CD86 and CD83 in monocytes from patient PAT-1. (B) Leukocytes were collected from HIV-infected patients and healthy subjects. Cells were stained with three different antibody panels. Then, CytoBacBone was used to merge the different cytometry profiles. SPADE analysis was then performed to isolate monocytes. The biplot representations show the co-expression of CD64 and TLR2, CD11b and CD32, and CD86 and CD83 in monocytes from patient PAT-1.