Table 2.
Antibodies and cell markers used to create combined mass cytometry profiles including 72 cell markers.
| Panel #A | Panel #B | Panel #C | ||||
|---|---|---|---|---|---|---|
| Metals | Antibodies | Clones | Antibodies | Clones | Antibodies | Clones |
| Pr141 | CD66 | TET2 | CD66 | TET2 | CD66 | TET2 |
| Nd142 | HLADR | L243 (G46-6) | HLADR | L243 (G46-6) | HLADR | L243 (G46-6) |
| Nd143 | CD3 | UCHT1 | CD3 | UCHT1 | CD3 | UCHT1 |
| Nd144 | CD64 | 10.1.1 | CD64 | 10.1.1 | CD64 | 10.1.1 |
| Nd145 | CD8a | 37006 | CCR7 | 150503 | CD209 | DCN47.5 |
| Nd146 | IL-6 | MQ2-13A5 | NF-ATC | 7A6 | CD62L | DREG-56 |
| Sm147 | Granzyme A | CTLA-3 | CD335 | 9 E 2 | CD45 | D058-1283 |
| Nd148 | IL-1β | H1b-98 | IL-4 | 7A3-3 | CD137 | 4B4-1 |
| Sm149 | CD14 | M5E2 | CD14 | M5E2 | CD14 | M5E2 |
| Nd150 | CD123 | 7G3 | CD123 | 7G3 | CD123 | 7G3 |
| Eu151 | IL-8 | NAPII | CD107a | H4A3 | - | - |
| Sm152 | CD16 | B73.1 | CD16 | B73.1 | CD16 | B73.1 |
| Eu153 | CD23 | M-L233 | CD154 | TRAP1 | CXCR3 (CD183) | 1C6 |
| Sm154 | CD86 | 2,331 (FUN-1) | CD86 | 2,331 (FUN-1) | CD86 | 2331 (FUN-1) |
| Gd155 | CD32 | 2 E1 | CD32 | 2 E1 | CD32 | 2 E1 |
| Gd156 | MIP-1β | D21-1351 | CD54 | LB-2 | CD56 | AF12-7H3 |
| Gd158 | IP-10 | 6D4 | IL-2 | N7.48A | ITGβ7 | FIB504 |
| Tb159 | TNF-α | MAb11 | - | - | Bcl-6 | K112-91 |
| Gd160 | IL-1α | 364/3B3-14 | CD69 | FN50 | CD83 | HB15E |
| Dy161 | CD141 | MAB3947 | CD141 | MAB3947 | CD141 | MAB3947 |
| Dy162 | IL-12 | C8,6 | Ki67 | B56 | CD279 | MIH4 |
| Dy163 | CD1c | AF5916 | CD1c | AF5916 | CD1c | AF5916 |
| Dy164 | CXCR4 | 12G5 | CD25 | BC96 | CD127 | HIL-7R-M21 |
| Ho165 | TLR2 | REA109 | CD11a | HI111 | IL-10R | 3F9 |
| Er166 | CCR5 | 3A9 | CD11b | ICRF44 | CD27 | M-T271 |
| Er167 | CD28 | CD28.2 | CD38 | AT1 | IgG2c | A23-1 |
| Er168 | CD11c | B-ly6 | CD11c | B-ly6 | CD11c | B-ly6 |
| Tm169 | IFN-α | LT27:295 | - | - | - | - |
| Er170 | CD45RA | T6D11 | IgG2b | 27-35 | CCL5 | 2D5 |
| Yb171 | IFN-γ | 25723 | IL-10 | JES3-9D7 | IgG1 K | MOPC-21 |
| Yb172 | CD4 | L200 | MyD88 | RB2101 | IL-2RA | 24212 |
| Yb173 | Granzyme B | GB11 | Granzyme B | GB11 | Granzyme B | GB11 |
| Yb174 | CD19 | HIB19 | CD19 | HIB19 | CD19 | HIB19 |
| Yb175 | IL-1RA | AS17 | - | - | IgG2a | R35-95 |
| Yb176 | MCP-1 | 5D3-F7 | Perforin | dG9-DTAG9 | nfKB | K10-892.12.50 |
| Ir191 | - | - | - | - | - | - |
| Ir193 | - | - | - | - | - | - |
The metal isotopes, markers, and antibody clones are indicated for each panel. Backbone markers are indicated in bold.