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. 2019 Aug 6;13:360. doi: 10.3389/fncel.2019.00360

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Copyright © 2019 Liu, Zhang, Liu, Tang, Shu, Bao, Zhang, Gu, Xu and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TMEM16A inhibition attenuated BBB disruption after ischemic stroke. (A) Evans blue staining and (B) IgG extravasation in sham-operated groups, MCAO-Saline groups and MCAO-Caccinh-A01 groups on day 3 after MCAO (bar = 1 mm). (C) Representative immunofluorescence staining of ZO-1, occludin and claudin-5 on day 3 after MCAO (bar = 50 μm). (D,E) Representative Western blotting results of TMEM16A, ZO-1, occludin, claudin-5, ICAM-1 and MPO on days 1 and 3 after MCAO. Values are the mean ± SEM. Two asterisks indicate P < 0.01 vs. sham-operated groups. One number sign indicates P < 0.05 and two number signs indicate P < 0.01 vs. MCAO-saline groups.