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. 2019 Aug 6;13:76. doi: 10.3389/fnana.2019.00076

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Copyright © 2019 Broide, Winzer-Serhan, Chen and Leslie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bright- and dark-field photomicrographs showing α7 nAChR mRNA expression in various non-neuronal tissues from E17 and P0 mouse pups. (A,B) Strong punctate expression of α7mRNA in the posterior region of the tongue (arrow heads). (D,E) Low expression of α7mRNA is observed in the nasal epithelium. (G,H) Moderate levels α7mRNA expression are observed in the predentin layer of the developing tooth bud. (J,K) α7 mRNA expression is shown in epithelial cells lining the aorta. Sections are counterstained with cresyl-violet. (C,F,I,L) Adjacent sections showing background labeling in each tissue with the sense riboprobe. A, aorta; CV, cardiac vessel; D, dentine; F, frenulum; NE, nasal epithelium; O, odontoblasts; P, palate; PD, predentin; T, tongue; TB, turbinate bone; V, ventricle. Space bar = 250 μm (A–C), 25 μm (D–I), 100 μm (J–L).

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