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Bright- and dark-field photomicrographs showing α7 nAChR mRNA expression in muscle tissue at E13 and E17. (A,B) High expression of α7 mRNA is observed in the inter-digit regions of the limb buds at E13. (D,E) Lower expression of mRNA is observed in these regions at E17. (C,F) Adjacent sections showing background labeling with the sense riboprobe in the limb bud region. C, cartilage. Space bar = 100 μm.
