TABLE 1.
α7 nAChR subunit mRNA expression in the developing central nervous system (CNS).
|
Age |
||||
| Region | E13 | E15 | E17 | P0 |
| Olfactory bulb | – | ++ | ++ | ++ |
| Olfactory tubercle | – | +++ | ++++ | +++ |
| Telencephalon | ||||
| Parietal Cortex | ||||
| Cortical plate | +++ | ++++ | ++++ | ++++ |
| Layer 6 | NA | NA | ++++ | +++ |
| Intermediate zone | NA | ++ | ++ | + |
| Ventricular zone | – | + | + | – |
| Hippo. formation | ++ | ++++ | ++++ | +++/++++ |
| Septum | – | – | + | + |
| Basal ganglia | + | + | +/++ | +/++ |
| Preoptic area | ++ | ++ | ++ | ++ |
| Diencephalon | ||||
| Thalamus | ||||
| Lateral | –/+ | +/+++ | +/++++ | +/+++ |
| Medial | –/+ | +/++ | +/+++ | +/+++ |
| Hypothalamus | –/+ | +/++ | +/+++ | +/+++ |
| Mesencephalon | ||||
| Lateral | – | + | +/++ | +/++ |
| Mediodorsal | + | + | + | ++ |
| Medioventral | – | +/++ | +/++ | +/++ |
| Pons | ||||
| Lateral | + | +/++ | +/++ | +/++ |
| Mediodorsal | +/+++ | +/++++ | +/++++ | +/++++ |
| Medioventral | + | +/+++ | +/+++ | +/+++ |
| Medulla oblongata | ||||
| Lateral | + | ++ | ++ | ++ |
| Mediodorsal | +/++ | ++/+++ | ++/+++ | ++/+++ |
| Medioventral | +/++ | ++/++++ | ++/++++ | ++/+++ |
| Cerebellum | NA | + | + | + |
| Spinal chord | ||||
| Dorsal | ++ | +++ | +++ | +++ |
| Ventral | ++/+++ | +++/++++ | +++/++++ | +++/++++ |
Intensity is based on silver grain counts: + (>10 grains/25 μm2), ++ (>20 grains/25 μm2), +++ (>50 grains/25 μm2), ++++ (>90 grains/25 μm2). A double quantitative score indicates signified lower levels of mRNA surrounding spots of more intense signal.