FIGURE 1.

CD163 interacts with MYH9 in PAMs regardless of PRRSV infection status. Input means initial extract, and IP Ex represents immunoprecipitated extracts. Lysed PAMs were immunoprecipitated with anti-MYH9 pAb to detect CD163 (A) and with anti-CD163 Mab to detect MYH9 (B). PAMs were infected with PRRSV strain JXA1 at 0.1 MOI for 48 hpi, lysed, and immunoprecipitated with anti-MYH9 pAb to detect CD163 (C) and with anti-CD163 Mab to detect MYH9 (D). (E) Membrane-associated MYH9 in PAMs was extracted using a Membrane Protein Extraction Kit and anti-MYH9 pAb was used to detect MYH9 distribution in whole cell lysates and cytoplasmic and membrane fractions. Antibodies against ATP1A1 and α-tubulin were used to normalize overall membrane or cytoplasm protein levels to allow comparisons of proteins for equivalent numbers of cells.