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. 2019 Aug 6;10:1815. doi: 10.3389/fmicb.2019.01815

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Copyright © 2019 Hou, Xue, Li, Nan, Zhang, Li, Zhao, Hiscox, Stewart, Wu, Wang and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombinant SRCR1-4 protein directly interacts with MYH9 in PAMs. (A) PAMs were incubated with SRCR1-4 or the same quantity of BSA. Cells were subjected to immunofluorescence staining of MYH9 (red) and SRCR1-4 (green) and cellular nuclei were counterstained with DAPI (blue). Co-localization of MYH9 and SRCR1-4 was visualized by confocal microscopy. (B) The membrane fraction of SRCR1-4 or BSA-preincubated PAMs was extracted using the Membrane Protein Extraction Kit followed by Western blot analysis to detect MYH9 expression. (C) PAMs were pre-incubated with SRCR1-4 or BSA. Cytoplasmic and membrane fractions were separated using a Membrane Protein Extraction Kit and then each fraction was immunoprecipitated with anti-MYH9 pAb to detect the interaction between MYH9 and recombinant SRCR1-4 protein.