Table 1.
Primers used in this study.
| Primers | Sequence (5′-3′) |
|---|---|
| Primers for ORF cloning | |
| npff-ORF-F | ATGGACACAGCTGCGGTGGT |
| npff-ORF-R | TTATTTCTTGCCGAATCTCT |
| npffr1-ORF-F | ATGGAGATACTGGACAACGT |
| npffr1-ORF-R | TCAGTTATCCCACGCCTGAT |
| npffr2-1-ORF-F | ATGGACCAGAATCTAATTCC |
| npffr2-1-ORF-R | CTAAATCTGAGACACCTTTTCC |
| npffr2-2-ORF-F | ATGAATGAAGGACTTGGGAA |
| npffr2-2-ORF-R | TCAAATAGACACTGCAGTCAC |
| Primers for synthesizing sense | |
| and antisense digoxigenin | |
| (DIG)-labeled riboprobes | |
| npff-ish-F | CGCATTTAGGTGACACTATAGAA GCGCTGCGGTGGTGACTCTTCTGG |
| npff-ish-R | CCGTAATACGACTCACTATAGGGAGACA ATCCTCCGACATTACCTGCCC |
| Primers for real-time PCR | |
| npff-qRT-F | GCTGCGGTGGTGACTCTTCTG |
| npff-qRT-R | TTGTTCGGACTGCCTTGGATGTG |
| npffr1-qRT-F | GGTCTACACGGCGGTTCT |
| npffr1-qRT-R | CAGCCAGTGGGCGAAA |
| npffr2-1-qRT-F | TATCGCCACCTCAAAGC |
| npffr2-1-qRT-R | TTCAGACCCAACTCCACTC |
| npffr2-2-qRT-F | CTTTCCTGGCTGCCTCTGTG |
| npffr2-2-qRT-R | GCCTTCTCCAGGTCCTCCAT |
| orx-qRT-F | TGCAGAGCCGACTCCACCAG |
| orx-qRT-R | CAGGCAGGAGCGTTGTGATGG |
| ss-qRT-F | GGTGCTTCTTGTGGCTTTG |
| ss-qRT-R | GAGGTCCTTGTCGTTGGTGA |
| npy-qRT-F | GAGGGATACCCGATGAAACCG |
| npy-qRT-R | CCTCTTTCCATACCTCTGTCTCG |
| lep-qRT-F | TGCAACTTTTAAGTGGGGGTA |
| lep-qRT-R | TGTTGTAACCCTCCAGCACGG |
| gas-qRT-F | TGCTAAGAGGGAGAAACTG |
| gas-qRT-R | TATCTCGCGTTCATCGTC |
| ghrl-qRT-F | ACACCTGTTTGCTGGTCTTTC |
| ghrl-qRT-R | ATGTGATGTGGTTGGCCTCTG |
| mtl-qRT-F | TGCTGATGAAGGAGCGAGAA |
| mtl-qRT-R | TCCACCATGTTCCACCTGAG |
| cck-qRT-F | TGCCAACTACAACCAACCT |
| cck-qRT-R | GCGTCGTCCAAAGTCCAT |
| Reference gene | |
| 18s-F | GGGTCCGAAGCGTTTACT |
| 18s-R | TCACCTCTAGCGGCACAA |