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. 2019 Aug 6;10:1006. doi: 10.3389/fpls.2019.01006

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Copyright © 2019 García-Bastidas, Van der Veen, Nakasato-Tagami, Meijer, Arango-Isaza and Kema.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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External and internal symptoms of ‘Grand Naine’ at six weeks after inoculation with Fusarium odoratissimum II-5 (Tropical Race 4). The panels (A–F) show the results of different inoculation methods (top panels); (A) DM soil, (B) DM Sand, (C) pouring method and their respective controls in panels (D–F). Panels J,K are the associated negative controls using resistant accessions cv. Rose (J) and ‘Pahang’ (K). Plants inoculated with chlamydospores (G) or Maize kernels (H), also developed similar symptoms but the latency period differs from that of conidial inoculations. Plants shown were challenged with the highest inoculum doses described for each method. The negative controls were ‘Grand Naine’ inoculated with race 1 using the dipping method (I) and the mock (L).