Figure 4.

Silencing of fat-5 and fat-7 genes rescued dopaminergic degeneration and related behaviors in the model of C. elegans expressing human mutated α-synuclein (A53T mutation). Graphical representation of (A) percentage of normal dopaminergic neurons (n = 25–30 animals per group). (B) Representative images of dopaminergic normal and abnormal neurons with outgrowths and blebs, magnification 40× and scale bar 100 μm and (C) basal slowing and (D) ethanol avoidance behaviors (n = 10–15 animals) in JVR203 animals fed on different genetic RNAi treatments (L4440, fat-5, fat-7, nhr-49 and tub-1). Empty vector L4440 was considered as control. The data represent the mean ± SEM with significant differences between the control and treatments at *p < 0.05, **p < 0.01 and ***p < 0.001. Each experiment was repeated three times.