Fig. 3.
(A) Dose response of VU0071063 and diazoxide on the pancreatic β-cell membrane potential (Vm). After impalement the islet was perifused in the absence of glucose (0 mM glucose) to obtain maximal activation and in the presence of 20 mM glucose to obtain maximal blockade of Kir6.2/SUR1. In 20 mM glucose, increasing (A) diazoxide or (B) VU0071063 concentrations were added and Vm measured at steady state responses. (C) Accumulated dose responses of the VU0071063 and diazoxide effects on the β-cell Vm. The Vm in the presence of 0 and 20 mM glucose was considered 0% and 100%, respectively. *P < 0.001 statistically significant difference between VU0071063 and diazoxide at same dose by two-way ANOVA. (D) Summary of data from three experiments showing β-cell response to VU0071063 and tolbutamide treatment. *P < 0.0001, statistically significant difference between VU0071063 and 20 mM glucose or 20 μM VU0071063 + 50 μM Tolbutamide.