Effects of VU0071063 on glucose-induced insulin secretion from mouse islets. (A) Thallium flux concentration-response curves showing activation of Kir6.2/SUR1 channels in response to VU0071063 but not 34MT (structure shown in inset) or DMSO. (B) VU0071063 inhibits glucose-induced insulin secretion in isolated mouse islets. Mouse islets were treated with 10 μM VU0071063, 34MT, or diazoxide in the presence of low (2 mM) or high (14 mM) glucose. VU0071063 inhibited glucose-induced insulin secretion to a greater extent than the equimolar diazoxide, whereas the 34MT did not have any effect on the insulin secretion. The experiment was performed on islets isolated from three different mice. *P ≤ 0.03 statistically significantly different from low glucose, #P ≤ 0.001 statistically significantly different from high glucose + 10 μM VU0071063, @P < 0.04 statistically significantly different from high glucose +10 μM 34MT.