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. 2019 Jul 25;20(3):2774–2782. doi: 10.3892/mmr.2019.10534

Figure 3.

Figure 3.

HK2 is a potential downstream target of miR-185. (A) Predicted target sequence of miR-185 in the 3′-UTR of HK2. (B and C) OS cells were transfected with wild type or mutant 3′-UTR of HK2 in the presence of miR-185. Luciferase assay was performed to confirm the binding between miR-185 and the 3′-UTR of HK2. **P<0.01, ***P<0.001 vs. miR-control. (D) U2OS and Saos-2 cells were transfected with miR-185 mimics or control miRNA, and the mRNA levels of HK2 were determined using RT-qPCR. **P<0.01 vs. miR-control. (E) Overexpression of miR-185 in OS cells suppressed the protein expression of HK2. (F) RT-qPCR was performed to evaluate the expression of miR-185 in paired OS and adjacent normal tissues. ***P<0.001. (G) Spearman's correlation test revealed the inverse correlation between the expression of miR-185 and HK2 in OS tissues. r=−0.6192, P<0.001. HK2, hexokinase 2; miR, microRNA; OS, osteosarcoma; RT-qPCR, reverse transcription-quantitative chain reaction; UTR, untranslated region.