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. 2019 Jul 23;20(3):2694–2702. doi: 10.3892/mmr.2019.10514

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effect of CFHR3 on the expression of proliferation- and apoptosis-related genes and proteins in Huh-7 cells. (A) Western blotting was performed to determine the protein expression levels of Ki67, survivin, active-caspase-3, Bax and Bcl-2. (B) Summary of the relative protein expression levels. (C) Reverse transcription-quantitative polymerase chain reaction was performed to determine the mRNA expression levels of Ki67, survivin, Bax and Bcl-2. Expression levels were normalized to GAPDH. Data are presented as the mean ± standard deviation from three independent experiments. *P<0.05, **P<0.01 vs. Con. AC-3, active-caspase-3; Bax, Bcl-2-associated X; Bcl-2, B cell lymphoma 2; CFHR3, complement factor H-related 3; Con, control; NC, negative control.