Table I.
Gene | Primer | Sequence |
---|---|---|
CFHR3 | Forward | 5′-TGGGCATTAGTCAAGAATACAGTAAAA-3′ |
Reverse | 5′-ATTAATGCCGCTTCAATATGACTTT-3′ | |
Ki67 | Forward | 5′-AAAGTGCCCAAGCCATAGA-3′ |
Reverse | 5′-CACCATTTGCCAGTTCCTC-3′ | |
Survivin | Forward | 5′-TCATCCTCACTGCGGCTGTC-3′ |
Reverse | 5′-AGGTCATCTCGGCTGTTCCTG-3′ | |
Caspase-3 | Forward | 5′-ACTGGACTGTGGCATTGAG-3′ |
Reverse | 5′-GAAACAATACATGGAATCTG-3′ | |
Bax | Forward | 5′-GCGAATTGGAGATGAACT-3′ |
Reverse | 5′-GTGAGCGAGGCGGTGAGGAC-3′ | |
Bcl-2 | Forward | 5′-GGTTGCCTTATGTATTTGTTTG-3′ |
Reverse | 5′-CCTCCGCAATGCTGAAAG-3′ | |
PI3K | Forward | 5′-ATGCCTGCTCTGTAGTGGTGG-3′ |
Reverse | 5′-CATTGAGGGAGTCGTTGTGC-3′ | |
Akt | Forward | 5′-TTGTCATGGAGTACGCCAACG-3′ |
Reverse | 5′-ACAGCCCGAAGTCTGTGATCTT-3′ | |
mTOR | Forward | 5′-CCAATCATTCGCATTCAGTCC-3′ |
Reverse | 5′-AACAAACTCATGTCCGTTGCTG-3′ | |
GAPDH | Forward | 5′-AATCTCATCACCATATTCCA-3′ |
Reverse | 5′-CCTGCTTCACCACCTTGTTG-3′ |
Akt, protein kinase B; Bax, Bcl-2-associated X; Bcl-2, B cell lymphoma 2; CFHR3, complement factor H-related 3; mTOR, mammalian target of rapamycin; PI3K, phosphoinositide 3-kinase.