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. 2019 Jul 3;20(3):2143–2150. doi: 10.3892/mmr.2019.10468

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Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — PINO treatment promotes the expression of differentiation-associated factors in MC3T3-E1 cells. (A) In order to assess the effects of PINO treatment on the differentiation of MC3T3-E1 cells, the mRNA levels of several osteoblast-specific factors (Col-I, ALP, OPN, Runx2, and BMP-2) were detected by RT-qPCR. (B) The protein levels of these genes were detected by western blotting. Each value was represented by the mean ± SEM (n=3). GAPDH was used as an internal control. *P<0.05 and **P<0.01 vs. the control group. PINO, pinoresinol; Col-I, collagen type I; ALP, alkaline phosphatase; OPN, osteopontin; Runx2, runt-related transcription factor 2; BMP-2, bone morphogenetic protein-2.