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. 2019 Jul 3;20(3):2143–2150. doi: 10.3892/mmr.2019.10468

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Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — PINO stimulates ALP activity and mineralization in MC3T3-E1 cells. (A) In order to verify the effects of PINO or E2 treatment on osteoblast differentiation, ALP activity detection was measured after treatment. (B) The mineralization of MC3T3-E1 cells was reflected by Alizarin red staining (magnification, ×200). Each value was represented by the mean ± SEM (n=3). *P<0.05 and **P<0.01 vs. the control group. PINO, pinoresinol; ALP, alkaline phosphatase; E2, estrogen.