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. 2019 Jul 3;20(3):2143–2150. doi: 10.3892/mmr.2019.10468

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Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — H89 co-treatment reverses the positive effects of PINO on the viability and migration of MC3T3-E1 cells. (A) H89 is an inhibitor of the cAMP/PKA signaling pathway. In order to confirm the involvement of the cAMP/PKA signaling pathway in the mechanisms of the promoting effects of PINO on osteoblast differentiation, MC3T3-E1 cells were co-treated with PINO and H89, and the changes in cell viability of each experimental group were assessed by CCK-8 kit. (B and C) The changes of migration ability were detected by wound healing assay (magnification, ×200). Each value was represented by the mean ± SEM (n=3). *P<0.05 and **P<0.01 vs. the control group; ^##P<0.01 vs. the PINO group.