Table I.

Primers for reverse transcription-quantitative PCR.

Gene name	Primer sequences
Col-I	F: 5′-GAGCGGAGTACTGGATCG-3′
	R: 5′-GCTTCTTTTCCTTGGGGTT-3′
ALP	F: 5′-GATCATTCCCACGTTTTCACATT-3′
	R: 5′-TTCACCGTCCACCACCTTGT-3′
OPN	F: 5′- GCCACAAGTTTCACAGCCAC-3′
	R: 5′-AAAATGCAGTGGCCGTTTGC-3′
Runx2	F: 5′-AGCGGCAGAATGGATGAGTC-3′
	R: 5′-ACCAGACAACACCTTTGACG-3
BMP-2	F: 5′-TGAGCAAAGTGCTTGCACAC-3′
	R: 5′-AGCCCCCTGGAAGGGATTAT-3′
GAPDH	F: 5′-AGGTCGGTGTGAACGGATTTG-3′
	R: 5′-GGGGTCGTTGATGGCAACA-3

Col-I, collagen type I; ALP, alkaline phosphatase; OPN, osteopontin; Runx2, runt-related transcription factor 2; BMP-2, bone morphogenetic protein-2; F, forward; R, reverse.