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. 2019 Jul 15;20(3):2571–2582. doi: 10.3892/mmr.2019.10500

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Effects of BQ and OQ on induced ROS-mediated apoptosis in AGS cells. (A) Cells were treated with 5-FU, BQ and OQ, and then the intracellular ROS levels were measured by flow cytometry (left panels) and mean fluorescence intensity (MFI) was quantitated (right panel). (B) Cells were treated with NAC for 30 min and then incubated with BQ and OQ for 24 h. The intracellular apoptosis levels were measured by flow cytometry. Data are expressed as the means ± standard deviation of the results from three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. 5-FU, 5-fluorouracil; BQ, 2-(butane-1-sulfinyl)-1,4-naphthoquinone; OQ, 2-(octane-1-sulfinyl)-1,4-naphthoquinone; ROS, reactive oxygen species; NAC, N-acetyl-L-cysteine.