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. 2019 Jul 1;20(3):2236–2244. doi: 10.3892/mmr.2019.10447

Figure 2.

Figure 2.

Overexpression of miR-330-5p suppressed cell viability, promoted cell apoptosis and induced cell cycle arrest. (A-F) U2OS and MG63 cells were transfected with 50 nM miR-330-5p mimics, inhibitors, mimics NC or inhibitor NC, and used for subsequent analyses. (A) Transfection efficiency was assessed by reverse transcription-quantitative polymerase chain reaction after transfection for 48 h; **P<0.01 vs. Blank group. (B) U2OS and (C) MG63 cell viability was measured by Cell Counting Kit-8 assay at 24, 48 or 72 h. (D) Cell apoptosis was detected by flow cytometry after transfection for 48 h. Flow cytometry was also performed to observe the cell cycle distribution in (E) U2OS and (F) MG63 cells after transfection for 48 h. Data represent the mean ± standard deviation of three independent experiments. *P<0.05 and **P<0.01 vs. mimics NC group. miR, microRNA; NC, negative control.