Skip to main content
. 2019 Jul 1;20(3):2236–2244. doi: 10.3892/mmr.2019.10447

Figure 5.

Figure 5.

miR-330-5p inhibited cell viability and induced cell apoptosis by targeting survivin. (A) U2OS and MG63 cells were transfected with 2 µg pcDNA-survivin or pcDNA-vector for 48 h, and the overexpression efficiency was detected by western blot analysis. (B) U2OS and MG63 cells were co-transfected with the 2 µg pcDNA-survivin and 50 nM miR-330-5p mimics for 48 h, and then used for analysis. Western blot analysis was used to detect the survivin protein level after 48 h of co-transfection. (C) Viability of U2OS and MG63 cells was measured by Cell Counting Kit-8 assay after 48 h of co-transfection. (D) Apoptosis was detected by flow cytometry after 48 h of co-transfection. (E) Migration ability of U2OS and MG63 cells, as measured by the wound healing assay after 48 h of co-transfection. (F) Invasion ability of U2OS and MG63 cells, as measured by the Transwell invasion assay after 48 h of co-transfection. Data represent the mean ± standard deviation of three independent experiments. *P<0.05 and **P<0.01 vs. Blank group in U2OS or MG63 cells; ##P<0.01 vs. miR-330-5p + pcDNA-vector group in U2OS or MG63 cells. miR, microRNA; NC, negative control.