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. 2019 Jul 12;20(3):2812–2822. doi: 10.3892/mmr.2019.10490

Figure 2.

Figure 2.

si-Zeb1 inhibits the growth and migration of HTR-8/sv neo cells. (A) Viability of HTR-8/sv neo cells transfected with scrambled siRNA or si-Zeb1 was assessed at different time points. (B) Flow cytometric analysis of cell cycle progression of HTR-8/sv neo cells transfected with scrambled siRNA or si-Zeb1 for 24 h. (C) Flow cytometric analysis of apoptosis of HTR-8/sv neo cells transfected with scrambled siRNA or si-Zeb1 for 24 h. The Q3 region contains early apoptotic cells; these data were used for statistical analysis. (D) Migration of HTR-8/sv neo cells transfected with scrambled siRNA or si-Zeb1 for 24 h (magnification, ×400). (E and F) Expression levels of Zeb1, E-cad, N-cad, VEGF, TRAIL-R2, TRAIL-R3, cyclin D1 and Bcl-2, as detected by western blot analysis and reverse transcription-quantitative PCR, in HTR-8/sv neo cells transfected with scrambled siRNA or si-Zeb1 for 24 h. All experiments were repeated in triplicate **P<0.05 compared with scrambled control. E-cad, E-cadherin; N-cad, N-cadherin; OD, optical density; si/siRNA, small interfering RNA; TRAIL-R, TNF receptor superfamily member; VEGF, vascular endothelial growth factor; Zeb1, zinc finger E-box-binding homeobox 1.