Table II.
Primers used for reverse transcription-quantitative PCR.
| Name | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| Zeb1 | GCACAACCAAGTGCAGAAG | CATTTGCAGATTGAGGCTG |
| E-cadherin | GGGGTCTGTCATGGAAGGTG | CGACGTTAGCCTCGTTCTCA |
| N-cadherin | CGGCCCGCTATTTGTCATCA | TGCGATTTCACCAGAAGCCT |
| VEGF | GCAAAAACGAAAGCGCAAG | GGAGGCTCCAGGGCATTAGA |
| TRAIL-R2 | CCACAAAGAATCAGGCATCA | CCAGGTCGTTGTGAGCTTCT |
| TRAIL-R3 | GATCGTCCCATCCCCACATC | CTGCTCTGACCAAGGCTGAA |
| Cyclin D1 | CCGAGGAGCTGCTGCAAATG | CGTGCGGGGTCATTGCGGC |
| Bcl-2 | CTTTGAGTTCGGTGGGGTCA | GAAATCAAACAGAGGCCGC |
| GAPDH | GAAGGCTGGGGCTCATTTG | AGGGGCCATCCACAGTCTTC |
All experiments were repeated at least in triplicate. mRNA expression levels were normalized to the internal reference gene GAPDH. Zeb1, zinc finger E-box-binding homeobox 1; VEGF, vascular endothelial growth factor; TRAIL-R, TNF receptor superfamily member.