Effects of miR-299-3p on cell invasion in vitro. (A) HGC-27 and SGC-7901 cells were transfected with miR-299-3p or Ctrl. *P<0.05, **P<0.01. (B) HGC-27 and SGC-7901 cells were transfected with anti-miR-299-3p or NC. **P<0.01, ***P<0.001. (C) Cells were seeded into Matrigel-coated invasion chambers 24 h post-transfection with miR-299-3p or Ctrl, and allowed to invade for 24 h. Magnification, ×200. Right panel, quantification of the invasion of cells through the Matrigel layer. *P<0.05. (D) Cells were seeded into Matrigel-coated invasion chambers 24 h post-transfection with anti-miR-299-3p or NC, and allowed to invade for 24 h. Magnification, ×200. Right panel, quantification of the invasion of cells through the Matrigel layer. *P<0.05, **P<0.01. miR-299-3p, microRNA-299-3p; Ctrl, scramble mimics; NC, non-targeting inhibitor control.