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. 2019 Jul 9;20(3):2258–2266. doi: 10.3892/mmr.2019.10477

Figure 2.

Figure 2.

Effect of MCM2 knockdown on A2780 cell proliferation, cell cycle and apoptosis. (A) Cell proliferation was assessed using the Cell Counting Kit-8 assay at 0, 1, 2, 3, 4 and 5 days after infection. Cell proliferation was inhibited by MCM2 knockdown at 4 and 5 days. (B) Cell cycle was analyzed by flow cytometry. Representative cell cycle histograms of A2780 cells are presented. Cells treated with MCM2 shRNA exhibited a higher number of cells in the G0/G1-phase. (C) Cell apoptosis was determined by flow cytometry. Infection with shMCM2 did not induce apoptosis in A2780 cells. A representative flow cytometry analysis is presented. Data are presented as the mean ± standard deviation from three experiments. *P<0.05 vs. shCON. MCM2, minichromosome maintenance complex component 2; shRNA, short hairpin RNA; shCON, control shRNA; shMCM2, shRNA targeting MCM2; OD, optical density.