Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 8;20(3):2091–2100. doi: 10.3892/mmr.2019.10474

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Lu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 3. — DP induces G2 phase cell cycle arrest in ESCC cells. (A and B) Flow cytometric analysis using PI staining showed that the cell cycle was blocked in the G2/M phase after treatment with DP (0, 40 and 80 µM) for 24 h in ECA109 and EC9706 cells. (C-F) A panel of G2/M phase-related protein was detected by western blot analysis. DP (0, 40, 60 and 80 µM) treatment for 24 h upregulated p21 and p27 expression and downregulated cyclin B1 and Cdc2 expression in a concentration- and time-dependent manner. The data are expressed as the mean ± standard deviation (n=3). *P<0.05 compared with the control group. DP, dracorhodin perchlorate; ESCC, esophageal squamous cell carcinoma; PI, propidium iodide.