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. 2019 Jul 1;20(3):2227–2235. doi: 10.3892/mmr.2019.10449

Figure 5.

Figure 5.

Silencing siah-1 suppresses Hcy-induced cell death. (A) Protein levels of p-p53 and total p53 were normalized with actin and then p-p53 was normalized to total p53. Hcy increased the expression of p-p53 and total p53 compared with the control cells. Following siRNA interference to silence the expression of siah-1, the increased expression of p-p53 and total p53 caused by Hcy was significantly inhibited. The knockdown of siah-1 also inhibited p53 phosphorylation at serine 15. (B) Hcy (8 mmol/l) significantly upregulated the protein expression of cleaved caspase 3, whereas siah-1 silencing with target siRNA significantly reduced the protein expression of cleaved caspase 3 in the Hcy-treated C6 cells. *P<0.05 vs. NC group. Hcy, homocysteine; siRNA, small interfering RNA; NC, negative control; p-, phosphorylated.