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. 2019 Jul 19;20(3):2743–2753. doi: 10.3892/mmr.2019.10508

Figure 3.

Figure 3.

Effect of BMP-9 overexpression on liver histology, serum ALT, serum AST and liver triglyceride levels in mice fed the MCD diet for 4 weeks. BMP-9 overexpressing and wild type mice, both with a C57BL/6 genetic background, were fed either the control diet or the MCD diet for 4 weeks. (A) Bar graphs presenting the results of the quantitative analysis of the mRNA expression of BMP-9 in the liver tissues of the 4 groups. (B) Western blotting of the protein expression of BMP-9 in the liver tissues of the 4 groups. (C) Representative photomicrographs of H&E-stained and (D) Oil Red-stained liver tissues for the 4 groups (magnification, ×400). (E) The NAS for the 4 different groups. (F) Bar graphs presenting the results of the quantitative analysis of serum AST, (G) serum ALT and (H) liver triglyceride levels in the 4 groups. *P<0.05, **P<0.01 and ***P<0.001, as indicated. Data are expressed as the mean ± standard error of the mean (n=6 mice in each group). NAFLD, non-alcoholic fatty liver disease; NAS, NAFLD activity score; BMP-9, bone morphogenetic protein-9; MCD, methionine- and choline-deficient diet; ALT, alanine aminotransferase; AST, aspartate transaminase; ns, not significant; H&E, hematoxylin and eosin.