Figure 6.

Effects of Let-7a inhibitor on the STAT3 signaling pathway. ATDC5 cells were treated with 5 µg/ml lipopolysaccharide for 5 h after 48 h of transfection with miR-NC inhibitor, Let-7a inhibitor or Let-7a inhibitor + IL-6R-siRNA. (A) Western blot assay was performed to quantify the protein expression level of p-STAT3 and IL-6R. (B) Densitometry analysis. Reverse transcription-quantitative PCR was used to detect the mRNA level of (C) IL-6R and (D) STAT3. Data are presented as the mean ± SD. **P<0.01. IL-6R, interleukin 6 receptor; p-, phosphorylated; siRNA, small interfering RNA; Let-7a, microRNA-let7a; NC, negative control; miR, microRNA.