Figure 11.

TLR4/MD-2-dependent staining of RAW264.7 cells with FITC-LTA. (A) RAW264.7 cells were treated with PBS, anti-mouse mAbTLR4/MD-2 complex (MTS510 Ab) or isotype control (IgG) for 1 h at 4°C before staining with 10 μg/mL. The cells were then harvested by trypsin and washed five times with PBS. The above cells were analyzed by the flow cytometry. (B) Cells were treated with PBS, MTS510 Ab, or IgG before FITC-LTA staining for confocal laser scanning microscopy analysis. Bar, 10 μm. Data are given as mean values ± standard deviation from 3 biological replicates. NS: P > 0.05, ^****P ≤ 0.0001.