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. 2019 Aug 6;10:1689. doi: 10.3389/fimmu.2019.01689

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Copyright © 2019 Toman, Celer, Kavanová, Levá, Frolichova, Ondráčková, Kudláčková, Nechvátalová, Salat and Faldyna.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell mediated immunity. The activity of blood lymphocytes was measured in the lymphoblast transformation test (LTT) using 5-day cultivation of cells. (A) Control, activity of non-stimulated cells. (B) Activity of cells stimulated with PRRSV antigen. The activities were measured after adding ³H-thymidine and counted as counts per minute (CPM) in a beta-counter. (C) Ratio the ratio of stimulated to non-stimulated cells (stimulating index—SI). (D) ELISPOT. The number of IFN-γ producing cells was calculated in cells from bronchoalveolar lavage fluid (BALF) on D63, i.e. 21 days after the challenge infection. The results were recalculated to the number of CD3⁺ lymphocytes.