Table 2.
Summary of immune responses and virus excretion of individual vaccines used in the experiment.
| Parameters | Time |
A1—inactivated (Progressis) |
A2—inactivated (Suivac) |
B3—mlv (Amervac) |
B4—mlv (Porcilis) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ANTIBODY PRODUCTION | ||||||||||||||
| Serum | Dynamics: IgM | D24 | + | + | – | – | ||||||||
| D28 | ++ | + | – | – | ||||||||||
| D35 | ++ | + | ++ | + | ||||||||||
| Dynamics: IgG | D28 | ++ | + | – | – | |||||||||
| D35 | ++ | + | ++ | ++ | ||||||||||
| D49 | ++ | + | ++ | ++ | ||||||||||
| D63 | + + + | + + + | ++ | ++ | ||||||||||
| Virus neutralization Ab | D42 | ± | ± | ++ | + | |||||||||
| D63 | + + + | + + + | + | ++ | ||||||||||
| Local response | Saliva IgG | D35 | + | – | – | – | ||||||||
| D49 | ++ | – | + | – | ||||||||||
| D63 | + + + | ++ | ++ | ++ | ||||||||||
| BALF IgG | D35 | + + + | + | + | – | |||||||||
| D63 | ++ | ++ | + | + | ||||||||||
| BALF IgA | D35 | ++ | ++ | ++ | + | |||||||||
| D63 | – | + | + | + | ||||||||||
| CELL MEDIATED IMMUNITY | ||||||||||||||
| LTT: non-stimulated cells | All | + + + | + + + | ++ | – | |||||||||
| LTT: stimulated cells (SI) | All | not evalable | not evaluable | + at D28 | ++ at D28, D42, D49, D63 | |||||||||
| IFNγ in Ag–stimulated subsets | D49 | positive CD4−CD8+cells | positive CD4−CD8+cells | positive CD4−CD8+cells | positive CD4−CD8+cells | |||||||||
| positive CD4+CD8+cells | positive CD4+CD8+cells | positive CD4+CD8+cells | positive CD4+CD8+cells | |||||||||||
| Elispot IFNγ | D63 | positive | positive | positive | positive | |||||||||
| VIRUS LOAD | ||||||||||||||
| serum | saliva | feces | serum | saliva | feces | serum | saliva | feces | serum | saliva | feces | |||
| Post immunization | D24 | – | – | – | – | – | – | ++ | ++ | – | ++ | ++ | – | |
| D42 | – | – | – | – | – | – | + + + | ++ | – | ++ | – | ++ | ||
| Post infection | D45 | ++ | ++ | + + + | + + + | – | + + + | + + + | ++ | + + + | + + + | ++ | + + + | |
| D56 | + | ++ | – | ++ | – | + | ++ | – | ++ | ++ | ++ | ++ | ||
| The end of experiment | D63 | + | ++ | ++ | + | ++ | + + + | ± | – | + | + | ++ | + | |
post vaccination (D0-D42) + + + positive (high); ++ positive (mid); + positive (low); ± positive in some animals.
post infection (D45-D63) –negative.
All intervals.
Groups of 5 piglets were immunized i.m with inactivated vaccine A1 (Progressis) or A2 (Suivac PRRS-In) at intervals D0 and D21. Groups of 5 piglets were immunized i.m with modified live vaccine B3 (Amervac PRRS) or B4 (Porcilis PRRS) at interval D21. All animals were infected with a challenge virus on D42, including the group of control non-immunized piglets (C5). All levels or activities after vaccination or infection are expressed as – (negative) or + to + + + as positive at different intensity.