TABLE 1.
BLASTn results for detected and ABSA-filtered human pathogens with kraken2 classified reads for each library kit applied for shotgun sequencing of a F. tularensis contaminated hare liver: Nextera DNA Flex (NDF), Nextera XT (NXT), ThruPLEX (TP) and TruSeq Nano (TSN).
| Species | NDF | NXT | TP | TSN |
| Francisella tularensis | 999/1000 | 992/1000 | 960/1000 | 998/1000 |
| Francisella philomiragia | 14/38 | 3/12 | 26/76 | 12/40 |
| Arcobacter butzleri | 1/22 | 0/0 | 0/0 | 0/0 |
| Streptococcus suis | 1/48 | 0/0 | 0/0 | 0/0 |
| Moraxella osloensis | 0/0 | 5/20 | 2/12 | 0 |
| Acanthamoeba castellanii | 1/50 | 0/0 | 0/0 | 0/0 |
| Atopobium parvulum | 1/2 | 0/0 | 0/0 | 0/0 |
| Streptococcus gallolyticus | 0/0 | 0/0 | 0/0 | 1/10 |
Numbers indicate how many reads could be verified with BLASTn from total number of kraken2 classified input reads.