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. 2019 Aug 6;10:1700. doi: 10.3389/fmicb.2019.01700

Figure 3.

Figure 3

Absence of CLP1 compromises induced (hemi)cellulase gene expression. (A) Hydrolytic zone formation of Δclp1, Reclp1, and TU6-RP strains on solid plate covered with a layer of 0.4% (w/v) ground Avicel. (B) Extracellular pNPC hydrolytic activities of the culture supernatant from Δclp1, Reclp1, and TU6-RP strains cultured on 1% (w/v) Avicel. (C) SDS-PAGE analysis of the culture supernatant from TU6-RP and Δclp1 strains cultured on 1% (w/v) Avicel. (D) Extracellular xylanase activities of the culture supernatant from Δclp1, Reclp1, and TU6-RP strains cultured on 0.5% (w/v) xylan. (E–I) Analyses of relative transcriptional levels of cel7a (E), cel7b (F), xyr1 (G), ace3 (H), and cre1 (I) in the Δclp1 and TU6-RP strains cultured on 1% (w/v) Avicel using quantitative RT-PCR. Significant differences (t test, *p < 0.05, **p < 0.01, ***p < 0.001) were observed in the phenotypes as indicated between the control strain TU6-RP and the Δclp1 strain. Values represent the mean of three biological replicates. Error bars are the SD from these replicates.