Skip to main content
. 2019 Aug 6;10:1700. doi: 10.3389/fmicb.2019.01700

Figure 5.

Figure 5

Subcellular localization of CLP1 and its mutants. (A,B) Subcellular localization of CLP1, the UIM-deleted mutant, and the PHD mutant with two point mutations (C145A and H150A) tagged with GFP at the C-terminus, respectively. Germinated hypha of T. reesei transformants of mCherry-h2b, mCherry-h2b&clp1-gfp, mCherry-h2b&clp1_ΔUIM-gfp, and mCherry-h2b&clp1_PHDM-gfp cultured on 1% (w/v) glucose or Avicel was subject to fluorescence analyses, respectively. (C) CLP1 is recruited to the cellulase gene promoters upon cellulose induction. ChIP analyses of CLP1 occupancy on the cellulase gene promoters of cel7a, cel7b, and the control gene actin in the Ptcu1-clp1-gfp and TU6-RP strains cultured on 1% (w/v) Avicel or glucose for 24 h. Significant differences (t test, **p < 0.01, *p < 0.05) were observed in the occupancy of CLP1 on the cel7a and cel7b promoters between the control TU6-RP and the Ptcu1-clp1-gfp strain under Avicel-inducing conditions. Values represent the mean of three biological replicates. Error bars are the SD from these replicates.