Skip to main content
. 2019 Aug 6;10:1700. doi: 10.3389/fmicb.2019.01700

Figure 6.

Figure 6

Overexpression of XYR1 fully rescued the defect in cellulase production but not conidiation resulting from clp1 deletion. (A,B) ChIP analyses of XYR1 occupancy on cel7a (A) and cel7b (B) promoters in the Δclp1 and TU6-RP strains after cultivation on 1% (v/v) glycerol or 1% (w/v) Avicel for 6 and 12 h. (C,D) Extracellular pNPC hydrolytic activities (C) and xylanase activities (D) of the culture supernatant from TU6-RP, Δclp1, and Δclp1&OExyr1 strains cultured on 1% (w/v) Avicel or 0.5% (w/v) xylan. (E,F) Conidia formation of the control strain TU6-RP, Δclp1, and Δclp1&OExyr1 strains incubated on malt extract agar plates at 30°C for 5 days (E) and conidia counting (F). Significant differences (t test, *p < 0.05, **p < 0.01, ***p < 0.001) were observed in the (hemi)cellulase production and conidiation between the control strain TU6-RP and the Δclp1&OExyr1 strain. Values represent the mean of three biological replicates. Error bars are the SD from these replicates.